After many Crime events have passed down to the small town of Huntsville in Alabama, you, the Detective, assume a role of a future 'Master Detective' in order to solve a series of crimes and discover the Big Boss behind the Criminal Organization "S.T.A.I.N.". Every Level, the Player must find all the clues in the list and discover the S.T.A.I.N. villains behind the crimes. At the last part of the Game, you will discover the S.T.A.I.N.'s Big Boss Gertrude Goodlittle, an old lady who manages the S.T.A.I.N. Once you've captured Gertrude, you will be ranked as a 'Master Detective' and continue your investigating career at the next game, Mystery Case Files: Prime Suspects.